{"id":121618,"date":"2022-05-18T14:05:13","date_gmt":"2022-05-18T08:35:13","guid":{"rendered":"https:\/\/www.mapsofindia.com\/my-india\/?p=121618"},"modified":"2022-05-18T14:05:13","modified_gmt":"2022-05-18T08:35:13","slug":"the-image-below-depicts-the-result-of-gel-electrophoresis","status":"publish","type":"post","link":"https:\/\/www.mapsofindia.com\/my-india\/education\/the-image-below-depicts-the-result-of-gel-electrophoresis","title":{"rendered":"The image below depicts the result of gel electrophoresis"},"content":{"rendered":"<h2>The image below depicts the result of gel electrophoresis<\/h2>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-121578 size-full\" src=\"https:\/\/www.mapsofindia.com\/ci-moi-images\/my-india\/2022\/05\/12-25.png\" alt=\"\" width=\"397\" height=\"280\" srcset=\"https:\/\/www.mapsofindia.com\/ci-moi-images\/my-india\/2022\/05\/12-25.png 397w, https:\/\/www.mapsofindia.com\/ci-moi-images\/my-india\/2022\/05\/12-25-150x106.png 150w, https:\/\/www.mapsofindia.com\/ci-moi-images\/my-india\/2022\/05\/12-25-300x212.png 300w, https:\/\/www.mapsofindia.com\/ci-moi-images\/my-india\/2022\/05\/12-25-100x70.png 100w\" sizes=\"auto, (max-width: 397px) 100vw, 397px\" \/><\/p>\n<h2>If the ladder represents sequence length upto 3000 base pairs (bp),<br \/>\na. Which of the bands (I &#8211; IV) correspond to 2500 bp and 100 bp respectively?<br \/>\nb. Explain the basis of this kind of separation and also mention the significance of this process.<\/h2>\n<h3>Answer.<br \/>\na. Band III corresponds to 2500 base pairs, and Band IV corresponds to 100bp.<br \/>\nb. The fragments will resolve according to their size. The shorter<br \/>\nsequence fragments would move farthest from well as seen in<br \/>\nBand IV (100 bp) which is lighter as compared to Band III which is<br \/>\nheavier being 2500 base pairs. The significance of electrophoresis is to purify the DNA fragments for use in constructing recombinant DNA by joining them with<br \/>\ncloning vectors.<\/h3>\n","protected":false},"excerpt":{"rendered":"<p>The image below depicts the result of gel electrophoresis If the ladder represents sequence length upto 3000 base pairs (bp), a. Which of the bands (I &#8211; IV) correspond to 2500 bp and 100 bp respectively? b. Explain the basis of this kind of separation and also mention the significance of this process. Answer. a. [&hellip;]<\/p>\n","protected":false},"author":21830,"featured_media":121571,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":{"0":"post-121618","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-education"},"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/posts\/121618","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/users\/21830"}],"replies":[{"embeddable":true,"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/comments?post=121618"}],"version-history":[{"count":1,"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/posts\/121618\/revisions"}],"predecessor-version":[{"id":121619,"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/posts\/121618\/revisions\/121619"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/media\/121571"}],"wp:attachment":[{"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/media?parent=121618"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/categories?post=121618"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.mapsofindia.com\/my-india\/wp-json\/wp\/v2\/tags?post=121618"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}