Why is insertional inactivation preferred over the use of selectable markers in cloning…

Why is insertional inactivation preferred over the use of selectable markers in cloning vectors?

Ans. Insertional inactivation is preferred over the use of selectable markers in cloning vectors because it allows for easy identification of recombinant plasmids without the need for additional selection steps. In insertional inactivation, the cloning vector contains a reporter gene interrupted by the insertion site for DNA fragments. Successful insertion of a DNA fragment disrupts the reporter gene, resulting in a loss of function that can be visually detected. This method simplifies the cloning process and eliminates the need for antibiotic selection, making it more convenient and cost-effective.