In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.

CBSE Sample Question Paper, Class 12 BIOLOGY Term 2 Question - In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.

In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.

Answer.
The flow chart shows the three steps involved in the process of PCR
showing the following
– Denaturation The DNA strands are treated with a temperature of
940C (Heat) and the strands are separated.
– Annealing The primers anneal to the complementary strands
– Extension The DNA polymerase facilitates the extension of the
strands.
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